Two-dimensional Electrophoresis of Soluble Proteins and Profile of Some Isozymes Isolated from Maize Plant in Response to Nacl

Maize genotype (Zea mays L) single cross 124 was grown in water culture in presence or absence of 150 mM NaCl for 15 days. Inhibition of plant growth and modification of plant morphology are the most sensitive responses of maize plant to salt stress. The growth of the 4 leaf 5,7,9,11 and 13 d after stress th application of NaCl was strongly reduced in comparison to their corresponding control. Electerophoretic analysis of total soluble protein (2-D PAGE or SDS-PAGE) and isozymes profiles were carried out in order to evaluate the response of maize genotype to salt stress. SDS-PAGE analysis has reveled that plant grown under NaCl showed induction or repression in the synthesis of few polypeptide in shoots and roots .In addition, proteins extracted from roots were analyzed by two-dimensional electrophoresis reveled two unique polypeptide to salt stress. Electerophoretic profiles of SOD (superoxide dismutase), APX (peroxidase) CAT (catalase), AP (Acid phosphates), EST (esterase) isozymes showed differences under salt stress. This differences reflected the biochemical adjustment of the plant to cope with the saline conditions. These results can be translated into efforts aimed to develop salt tolerant genotypes and maximiz the use of saline soils.